![]() ![]() In turn, APPL is regulated by direct interactions with the PDZ (postsynaptic density-95/Discs large/zona occludens-1)-containing protein dX11/Mint/Lin-10, which also regulates synapse expansion downstream of FasII. We show that the ability of FasII to stimulate or to prevent synapse formation depends on the symmetry of transmembrane FasII levels in the presynaptic and postsynaptic cell and requires the presence of the fly homolog of amyloid precursor protein (APPL). Here, we used the Drosophila larval neuromuscular junction to investigate the pathways activated by Fasciclin II (FasII), a transmembrane CAM of the Ig superfamily, during synapse remodeling. However, the downstream pathways activated by CAMs have remained mostly unknown. doi: 10.1016/j.cej.2017.11.030.Cell adhesion molecules (CAMs) have been universally recognized for their essential roles during synapse remodeling. Immobilization of cellulase on styrene/maleic anhydride copolymer nanoparticles with improved stability against pH changes. Wang Y., Chen D., Wang G., Zhao C., Ma Y., Yang W. Facile fabrication of electrochemical ZnO nanowire glucose biosensor using roll to roll printing technique. Immobilization of lipase onto novel constructed polydopamine grafted multiwalled carbon nanotube impregnated with magnetic cobalt and its application in synthesis of fruit flavours. Nanobiocatalysis and its potential applications. Identification of a multi-enzyme complex for glucose metabolism in living cells. Kohnhorst C.L., Kyoung M., Jeon M., Schmitt D.L., Kennedy E.L., Ramirez J.…An S. Such mega-enzyme complexes promise wider applications in the field of biotechnology and bioengineering.īinding modules Dockerin-cohesin interactions Multi-enzyme complex Protein scaffolds SpyTag-Sp圜atcher system. Various analytical and characterization tools that have enabled the development of these scaffolding strategies are also reviewed. Moreover, different conjugation strategies viz dockerin-cohesin interaction, SpyTag-Sp圜atcher system, peptide linker-based ligation, affibody, and sortase-mediated ligation are discussed in detail. This review describes the components of protein scaffolds, different ways of constructing a protein scaffold-based multi-enzyme complex, and their effects on enzyme kinetics. The scaffolding improves the catalytic performance, enzyme stability and provides an optimal micro-environment for biochemical reactions. With this respect, scaffolding proteins play an immense role in bringing different enzymes together in a specific manner. However, operating different enzymes together in a single vessel limits their operational performance which needs to be addressed. The synthesis of complex molecules using multiple enzymes simultaneously in one reaction vessel has rapidly emerged as a new frontier in the field of bioprocess technology. ![]()
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